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. 2015 Jun 20;6(27):23890–23904. doi: 10.18632/oncotarget.4341

Figure 1. Microvascular density (MVD) in mouse mammary tumors with Ncoa1 knockout or overexpression.

Figure 1

a. Detection of CD31-positive endothelial cells by immunohistochemistry in mouse mammary tumor tissue sections prepared from Tg(MMTV-PyMT), Tg(MMTV-PyMT) × Ncoa1−/−, Tg(MMTV-TVA/RCAS-PyMT), Tg(MMTV-TVA/RCAS-PyMT) × Tg(MMTV-NCOA1), Tg(MMTV-Neu) and Tg(MMTV-Neu) × Tg(MMTV-NCOA1) mice as indicated. Tumors were isolated from mice after palpable tumors were detected for the time in weeks indicated. Scale bar: 50 μm. b. Semi-quantitative analysis of MVD. The total number of microvessels in 5 different viewing fields of 200× magnification under a microscope was counted for each tumor section. Sections from at least 10 tumors in each group were examined. Data are presented as Mean ± SD. *p < 0.05 by Student's t test. c. QPCR analysis of CD31 mRNA in the mouse mammary tumors (n = 5) isolated from mice with the indicated genotypes. PyMT, Tg(MMTV-PyMT); TVA/PyMT, Tg(MMTV-TVA/RCAS-PyMT); Neu, Tg(MMTV-Neu); WT, wild type; NCOA1, Tg(MMTV-NCOA1).