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. 2015 Jun 19;6(27):23930–23943. doi: 10.18632/oncotarget.4525

Figure 4. Effect of shRNA-mediated repression of HPV16 E6 and E7 on DNA methyltransferase expression.

Figure 4

Immunoblot analysis of DNMT1, DNMT3A, DNMT3B and DNMT3L protein levels in SiHa (A and B) and in CaSki (C and D) were shown above. Protein extracts were made from uninfected (Mock) CaSki and SiHa cells, and from cells infected for 48 and 96 h with lentivirus expressing E6-, E7- and control- shRNA. Extracted protein samples (50μg per lane) were subjected to electrophoresis and immunoblotting with antibodies specific for the 4 protein and β-actin as control for equal loading.