Skip to main content
. 2015 Jun 3;6(27):24105–24118. doi: 10.18632/oncotarget.4152

Figure 3. A high-fat diet reduces DNA methylation levels in the miR-210 promoter in vivo.

Figure 3

A. CpG island (gray color) and HIF-1α-binding site predictions in the mouse miR-210 gene promoter. Suitable primers were designed to amplify the CpG region containing the HIF-1α-binding site. B. The high-fat diet effects on DNA methylation in the mouse miR-210 gene promoter. The arrow indicates the HIF-1α-binding site. The N1 ∼ 3 and N4 ∼ 6 respectively means the serial number of control and high-fat diet-fed mouse. Each row and circle respectively means one single sequencing reaction and one CpG site. The empty and solid circles respectively mean the un-methyl and methyl CpG site. C. Quantitative results for. After being fed a high-fat diet, genomic DNA was extracted from the aorta of an APOE−/− mouse. The methylation status was measured by the BSP assay. D. The high-fat diet effects on miR-210 levels. Total RNA was extracted from aortas of mice fed a chow or high-fat diet, and miR-210 levels were measured by a qPCR. Data are means ± SD of three experiments. *P < 0.05.