Figure 1. ΔNp63 downregulation is an early event in spontaneous Trp53-deficient epidermal tumors.

A–D. Representative examples of p63 expression in non lesional epidermis (A), dysplasia (B), moderately differentiated tumor (C) and undifferentiated tumor from Rb1^ΔEpi;Trp53^ΔEpi mice as assessed by immunohistochemistry. E–F. Summary of p63 expression in samples determined by immunohistochemistry (E) or qRT-PCR (F) G. Immunoblot of the quoted protein extracts showing the expression of p63 isoforms. Note the lack of ΔNp63α expression in tumors, regardless their genotype, and the presence of a fast migrating isoform in some of them (denoted by red arrow). H, H'. Representative examples of p63 expression in lung metastases. H' is a high magnification of the area denoted in H covering the metastatic nodule. Bars = 150 μm