Figure 3. C18-Cer and dhC16-Cer induce accumulation of ceramides and apoptosis for PEL cells.

A.–C. BCBL-1 cells were incubated with the indicated concentrations of C18-Cer, dhC16-Cer or vehicle for 24 h, then apoptosis A.–B. and protein expression C. quantified as in Methods. D. BCBL-1 cells were incubated with C18-Cer (40 μM), dhC16-Cer (40 μM) or vehicle for 24 h, then intracellular ceramide and dh-ceramide species were quantified as described in Methods. Error bars represent the S.E.M. for 3 independent experiments, * = p < 0.05; ** = P < 0.01.