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. 2015 Jul 3;6(27):24246–24260. doi: 10.18632/oncotarget.4759

Figure 4. C18-Cer and dhC16-Cer induce expression of ceramide synthases within PEL cells.

Figure 4

A.–B. BCBL-1 cells were incubated with C18-Cer (40 μM), dhC16-Cer (40 μM) or vehicle for 24 h, then transcript A. and protein B. expression of CerS isoforms quantified using qRT-PCR and immunoblots, respectively. C.–D. Cells were transfected with control non-target siRNA (n-siRNA) or CerS2-siRNA for 48 h, then incubated with C18-Cer (40 μM), dhC16-Cer (40 μM) or vehicle for 24 h. Protein expression was detected by immunoblots, and cell apoptosis quantified as above. Error bars represent the S.E.M. for 3 independent experiments, * = p < 0.05; ** = P < 0.01.

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