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. 2015 Jul 3;6(27):24246–24260. doi: 10.18632/oncotarget.4759

Figure 5. C18-Cer and dhC16-Cer induce KSHV lytic gene expression.

Figure 5

A.–C. BCBL-1 cells were incubated with C18-Cer (40 μM), dhC16-Cer (40 μM) or vehicle for 24 h, then qRT-PCR used to quantify representative KSHV latent (ORF73) and lytic transcripts (ORF50, ORF74, K8.1, ORF57). Expression of the viral lytic protein K8.1 was determined using immunoblots and IFA. D.–E. BCBL-1 were transfected with control n-siRNA or ORF50-siRNA for 48 h, then incubated with dhC16-Cer (40 μM) or vehicle for 24 h. Viral gene expression and cell apoptosis were quantified by qRT-PCR and flow-cytometry, respectively. F. BCBL-1 were transfected with control n-siRNA or CerS2-siRNA for 48 h, then representative viral transcripts quantified by qRT-PCR. Error bars represent the S.E.M. for 3 independent experiments, * = p < 0.05; ** = P < 0.01.

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