Skip to main content
. 2015 Jul 3;6(27):24246–24260. doi: 10.18632/oncotarget.4759

Figure 8. Short-chain ceramides induce expression of ceramide synthases within PEL cells.

Figure 8

A.–B. BCBL-1 cells were incubated with C2-Cer (50 μM), C6-Cer (6.25 μM), C8-Cer (12.5 μM) or vehicle for 24 h, then transcript A. and protein B. expression of CerS isoforms quantified using qRT-PCR and immunoblots, respectively. C.–D. Cells were transfected with control n-siRNA, CerS2-siRNA or CerS6-siRNA for 48 h, then incubated with C6-Cer (6.25 μM) or vehicle for 24 h. CerS protein expression and cell apoptosis were determined as above. Error bars represent the S.E.M. for 3 independent experiments, * = p < 0.05; ** = P < 0.01.

HHS Vulnerability Disclosure