Figure 9. C6-Cer suppresses PEL formation and induces regression of established PEL tumors in vivo.

A.–C. NOD/SCID mice were injected i.p. with 10⁷ BCBL-1 cells. Beginning 24 h later, 20 mg/kg C6-Cer or vehicle (n = 10 per group) were administered i.p. 3x/week, for each of 2 independent experiments. Weights were recorded weekly. Images of representative animals and their respective spleens, as well as ascites fluid volumes, were collected at the conclusion of experiments on day 28. Error bars represent the S.E.M. for 2 independent experiments, ** = p < 0.01. D. Spleens from representative vehicle- or C6-Cer-treated mice were prepared for routine H&E staining. E. Immunoblots were used to detect CerS protein expression within splenic lysates from representative vehicle- or C6-Cer-treated mice. F.–H. NOD/SCID mice were injected i.p. with 10⁷ BCBL-1 cells. Beginning 28 days later, 20 mg/kg C6-Cer or vehicle (n = 10 per group) were administered i.p. 3x/week, for an additional 21 days for each of 2 independent experiments. Weights were recorded weekly, and images of representative animals and their respective spleens, as well as ascites fluid volumes, were collected at the conclusion of experiments on day 49.