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. 2015 Jun 10;6(27):24463–24473. doi: 10.18632/oncotarget.4425

Figure 1. Schematic diagram of translational immunoprecipitation (TrIP-chip) approach to identify miRNA translationally regulated targets.

Figure 1

Cycloheximide treated polyribosome complexes are cross linked to mRNA via DSP. Cells are then lysed and the polyribosome/mRNA complex is immunoprecipitated by targeting HSP70 using anti-HSP70 antibody conjugated to magnetic beads. mRNA is isolated from the complex using Array Pure Nanoscale RNA Purification Kit. mRNA expression levels can then be assessed by- microarray.