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. Author manuscript; available in PMC: 2015 Dec 29.
Published in final edited form as: Bioorg Med Chem Lett. 2013 Apr 10;23(11):3406–3410. doi: 10.1016/j.bmcl.2013.03.076

Figure 1.

Figure 1

Activity of endogenously-expressed sialidases in HL60 against N-acyl neuraminic acid glycosides 9a-9f. Whole HL60 cells in acetate buffer (pH 4.5) were treated for 2 h with 0.125 mM of each compound (blue bars) at 37°C. Grey bars represent the same reaction in the presence of 0.1 mM of sialidase inhibitor DANA. The pH of each reaction was adjusted to 10.7 with glycine buffer and fluorescence was read with excitation wavelength of 365 nm and emission wavelength of 450 nm. Error bars are the standard deviations. All experiments were performed in at least biological triplicate and are representative of multiple independent trials.