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(a) H&E staining of the scaffold on days 3, 14 and 28 indicate enlargement of pellets inside the scaffold, (b) High magnification images at different time points of day 3, 14, and 28 demonstrate cells round morphologies which over time spaces between the cells increase and filled with extra cellular matrix, (c) Masson-trichrome staining (blue color) illustrates increasing collagen content over 28 days of culture with increasing the blue color intensity.
