Fig. 5.

GST-4::GFP expression is induced by juglone and suppressed by the C2 organic selenium compound.

Worms were pre-treated with each of the compounds for 1 h (200 μM), washed three times in M9 and subjected to juglone treatment (200 μM) for 1 h. GST-4::GFP fluorescence was microscopically evaluated with an Olympus IX81 Inverted Microscope at 20× magnification, using the green filter for fluorescence (A) and with the SpectraMax® i3 Multi-Mode Microplate Reader (B) # represents statistically significant (p < 0.001) difference compared to control and * indicates statistically significant (p < 0.05) difference compared to the juglone treated group. Analysis was carried out by means of one-way ANOVA followed by Newman–Keuls Multiple Comparison Test (mean ± SEM). Twenty animals were observed in four independent experiments.