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. Author manuscript; available in PMC: 2016 Mar 17.

Published in final edited form as: Crit Rev Anal Chem. 2016;46(2):93–105. doi: 10.1080/10408347.2014.980775

Note: A standard solution of 3-PBA in MeOH was infused into a QQQ 6490 LC-MS/MS system (Agilent Technologies, Waldbronn, Germany) using a Fusion 100 syringe pump (Chemyx Inc., Stafford, TX) at a flow rate of 0.1 ml/min via T-connector. The LC and MS modules were programmed and controlled using Mass Hunter Software version B.04.01 (Agilent Technologies). An isocratic composition of water (10%) and methanol (90%) (with 0.1% acetic acid) was supplied by the HPLC pump at a flow rate of 0.2 ml/min. The MS/MS parameters were set as follows: ESI (negative mode); 300 °C drying gas temperature; 325 °C sheath gas temperature; 5 L/min drying gas flow; 10 L/min sheath gas flow; 35 psi nebulizer pressure; 3000 V capillary voltage; 500 V nozzle voltage; 250 msec dwell time; and 400 V delta EMV (−). Nitrogen was used as collision gas. Monitoring MS/MS transition for 3-PBA was 21393 @ 16 CE. The injection volume of sample extractant was 5 μL.

Inline graphic — Note: A standard solution of 3-PBA in MeOH was infused into a QQQ 6490 LC-MS/MS system (Agilent Technologies, Waldbronn, Germany) using a Fusion 100 syringe pump (Chemyx Inc., Stafford, TX) at a flow rate of 0.1 ml/min via T-connector. The LC and MS modules were programmed and controlled using Mass Hunter Software version B.04.01 (Agilent Technologies). An isocratic composition of water (10%) and methanol (90%) (with 0.1% acetic acid) was supplied by the HPLC pump at a flow rate of 0.2 ml/min. The MS/MS parameters were set as follows: ESI (negative mode); 300 °C drying gas temperature; 325 °C sheath gas temperature; 5 L/min drying gas flow; 10 L/min sheath gas flow; 35 psi nebulizer pressure; 3000 V capillary voltage; 500 V nozzle voltage; 250 msec dwell time; and 400 V delta EMV (−). Nitrogen was used as collision gas. Monitoring MS/MS transition for 3-PBA was 21393 @ 16 CE. The injection volume of sample extractant was 5 μL.