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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Neurosci Lett. 2015 Oct 28;610:79–85. doi: 10.1016/j.neulet.2015.10.054

Fig. 2.

Fig. 2

Plasma membrane expression of ERα in pure astrocyte cultures. Western blot analysis of Na2CO3 sucrose density gradient fractions (2–9) insoluble in 1% Triton X-100. Insoluble product was collected by centrifugation, and resolved by SDS–PAGE and Western blot along with the crude soluble fraction (S). Flotillin-1 served as a control for fraction contamination and was appropriately expressed in fractions 4–6.