Figure 6. vHP CA1 neurons provide input to dpLHA ORX-expressing neurons.
(A) A representative PHAL (blue) injection site centered in ventral hippocampus field CA1. CA1sp, CA3sp: CA1 and CA3 pyramidal cells. (B) Darkfield microscopy, where neuroanatomical analyses were performed in the dpLHA (fx: fornix; V3h: Third ventricle; location shown in C). Inset (C) confocal imaging reveals some orexin neurons (red) in the dpLHA that are in close apposition to vHP field CA1-originating axons, labeled with PHAL (blue). (D) Triple-label imaging reveals co-labeling of PHAL and synaptophysin (green) in axons in apposition to dpLHA orexin-expressing soma (indicated by white arrows).
Figure 6—figure supplement 1. CA1 vHP neurons project ipsilaterally to the dpLHA.
(A–C) PHAL-immunoreactive axons originating from the vHP (field CA1) within the dpLHA, revealing an exclusively ipsilateral projection from the vHP to the dpLHA (v3h = 3rd ventricle; fx = fornix).