Figure 3. PPN1 neurons are courtship-promoting neurons in proximity to PP23 axons and P1 fibers.
(A) PPN1 neurons have cell bodies in the third leg neuromere of the VNC and send projections to the six leg neuromeres and wing neuromere of the VNC and to the ventrolateral protocerebrum of the brain. R56C09-Gal4 drives expression of UAS-CD8::GFP exclusively in the pair of PPN1 neurons. (B-C) PPN1 has dendrites in the VNC (B, DenMark, magenta) and axons in the ventrolateral protocerebrum (C, syt-GFP, green). B and C are from the same animal containing R56C09-Gal4, UAS-DenMark, UAS-synaptotagmin-GFP. (D) Activation of PPN1 with dTRPA1 causes increased male-female courtship at 30°C; mean ± SEM, n = 16–30/condition, **p<0.01 (2-way ANOVA, Bonferroni post-hoc). (E) Overlap is observed in the VNC between PPN1 dendrites (green) and incoming PPK25 axons (magenta). R56C09-LexA, lexAop-CD2::GFP, ppk25-Gal4, UAS-CD8::tdTomato flies were used. F. Overlap between PPN1 (green) and P1 (magenta) in the anterior ventrolateral protocerebrum (50 μm collapsed Z-stack). Scale bars, 25 μm (F) 50 μm (A-E).