Fig. 6. The gene expression of epithelium-derived cytokines in ALI culture of NHNE and ARNE cells. The mRNA levels of TSLP, IL-25, and IL-33 were measured in ALI culture of NHNE and ARNE cells 14 days after confluence. (A) Real-time PCR showed that the TSLP mRNA level was significantly higher in ALI culture of ARNE cells. However, IL-25 (B) and IL-33 (C) mRNA levels were not significantly different between NHNE and ARNE cells (White dot, NHNE cells; Black dot, ARNE cells). Results are presented here as the mean±SD from eight independent ALI cultures (^*P<0.05 compared to mRNA levels in NHNE cells).