Fig. 1. Relative gene expression of two 5α-reductase (5αR) isoforms in cultured human dermal papilla cells (DPCs). (A) Relative gene expression of 5αR1 and 5αR2 were detected using quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) in DPCs. Data represent three independent experiments and relative expression values were calculated using the equation RQ=2^-ΔΔct. (B) qPCR analysis of 5αR1 and 5αR2 mRNA expression in DPCs following herbal extract treatment using graded concentrations. Expression levels were normalized to human glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA. Data are mean±standard deviation; ^*p<0.05 and ^†p<0.01 compared with untreated control group.