Table 2.
MLVA & MLST Primers and their references
| MLVA Loci (Alias) | Range of Amplicon Size | Primer Sequence (5′-3′) | References |
|---|---|---|---|
| SE2 (SENTR6, ENTR20) | 208–229 | F-CTTCGGATTATACCTGGATTG R-TGGACGGAGGCGATAG |
Cho et al. 2008 |
| SE3 | 308–320 | F-CAACAAAACAACAGCAGCAT R-GGGAAACGGTAATCAGAAAGT |
|
| SE5 (SENTR5, STTR5) | 200–224 | F-CGGGAAACCACCATCAC R-CAGGCCGAACAGCAGGAT |
|
| SE7 | 484–545 | F-CCGACCCAATAAGGAG R-CTTACCGTTGGTAGTTTGTTA T |
|
| SE8 | 469–556 | F-TTGCCGCATAGCAGCAGAAGT R-GCCTGAACACGCTTTTTAATAGGCT |
|
| SENTR4 (SE1, ENTR13) | 119–126 | F-GACCAACACTCTATGAACCAATG R-ACCAGGCAACTATTCGCTATC |
Malorny et al. 2008 |
| SENTR7 (SE9) | 126–135 | F-ACGATCACCACGGTCACTTC R-CGGATAACAACAGGACGCTTC |
| MLST Loci (Alias) | Data Base Standard Size | Primer Sequence (5′-3′) | References |
|---|---|---|---|
| thrA (aspartokinase+homoserine dehydrogenase) | 501 | F-GTCACGGTGATCGATCCGGT R-CTCCAGCAGCCCCTCTTTCAG |
Achtman-1 et al. 2015 |
| purE (phosphoribosylaminoimidazole carboxylas) | 399 | F-CGCATTATTCCGGCGCGTGT R-GAACGCAAACTTGCTTCAT |
|
| sucA (alpha ketoglutarate dehydrogenase) | 501 | F-AGCACCGAAGAGAAACGCTG R-GGTTGTTGATAACGATACGTAC |
|
| hisD (histidinol dehydrogenase) | 501 | F-GTCGGTCTGTATATTCCCGG R-GGTAATCGCATCCACCAAATC |
|
| aroC (chorismate synthase) | 501 | F-CCTGGCACCTCGCGCTATAC R-CCACACACGGATCGTGGCG |
|
| hemD (uroporphyrinogen III cosynthase) | 432 | F 5′-GTGGCCTGGAGTTTTCCACT R-GACCAATAGCCGACAGCGTAG |
|
| dnaN (DNA polymerase III beta subunit) | 501 | F-CCGATTCTCGGTAACCTGCT R-CCGCGGAATTTCTCATTCGAG |