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. 2015 Dec 8;30(1):37–47. doi: 10.1210/me.2015-1166

Figure 4.

Figure 4.

Sucrose density fractionation and immunoblot analyses of SgIII-shRNA and Ctrl-shRNA cells. Western blot analysis of Ctrl-shRNA (A) and SgIII-shRNA (B) cells showing POMC predominantly present in fraction numbers 10–15 from sucrose density fractionation of postnuclear supernatants. Note the shift and increase in levels of POMC to fraction number 9 in the SgIII-shRNA cells that cofractionated with syntaxin 6, a TGN marker (compare boxed areas). This experiment was performed on 2 different Ctrl-shRNA and 2 different SgIII-shRNA clonal cell lines on 2 occasions with similar results.