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. 2015 Dec 9;30(1):13–25. doi: 10.1210/me.2015-1075

Figure 1.

Figure 1.

Androgen down-regulates TM4SF3 mRNA and up-regulates the protein. LNCaP cells were treated with either ethanol (−) or 1nM R1881 (+) and measured for expression of TM4SF3 (A) mRNA by Q-RT-PCR or (B) protein by Western blotting. CDS primers were used to measure TM4SF3 mRNA expression. LNCaP cells were transfected with (C) control or TM4SF3 siRNA or (D) empty vector or TM4SF3-Flag vector, and protein expression was measured by Western blotting using an anti-TM4SF3 or anti-Flag antibody. The Student's t test was performed to show statistical significance (P < .05), as indicated by asterisks. Note that β-actin (B–D) was used as a loading control. For B–D, the numbers above the gel represent average quantifications of 3 TM4SF3 Western blottings, standardized to β-actin, and relative to the first lane, which was set to 1.