Figure 6.

Gene ontology analysis using the list of RNA-Seq identified genes differentially expressed between vehicle and dex-exposed male and female hypothalamic NPSCs determined the top functional pathway as proliferation of cells (A). Flow cytometric analysis of BrdU incorporation in male and female hypothalamic NPSCs treated with either vehicle or dex (B). Quantification of BrdU+ cells showed a significant overall main effect in a reduction in proliferation after dex treatment in both male and female hypothalamic NPSCs (P < .001) and post hoc analysis showed a female-specific reduction in proliferation after dex treatment compared with vehicle (C) (P < .01).