Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Dec 16;2016:4304916. doi: 10.1155/2016/4304916

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 Yan-Chuang Han et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Feeder-free SMINS-TTF-3 cells (FF SMINS3). TTF were seeded on PDL coated dishes in stem cell medium containing Bix01294, RG108, and PD0325901 for two weeks and then transferred into NSC medium in petri dishes for two weeks. (a) The FF SMINS3 cells were stained by neural stem cell markers Sox2 and Nestin. (b–f) The FF SMINS3 cells differentiated to astrocytes (GFAP), neurons (Tuj1, Map2, and NeuN), and oligodendrocytes (Olig2 and O4) in specific differentiation medium. DAPI was used for nuclei counterstaining (blue). Scale bar: 100 μm (a) and 50 μm (b–f).