Figure 1.
Effects of oxygen levels on long-term in vitro culturing and colony forming capacity of p53 wild-type mMSCs. (a) Proliferation and morphology of mMSCs p53wt/wt cultured in hypoxic conditions (2% O2, left) and in normoxic conditions (21% O2, right). Representative images show mMSCs cultured for 32 days. (b) Colony forming ability of mMSCs in hypoxic (upper) and normoxic (lower) atmospheres 14 days after plating 5,000 cells in a 75 cm2 flask. (c) Plating efficiency calculated as mean values of fibroblast colony forming units (CFU-F) per plated cell ± standard error of the mean (n = 4) in hypoxic (grey bar) and normoxic (black bar) conditions.