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Biofilms of P. aeruginosa PA01 harboring the green fluorescence protein (GFP) expressing vector pMRP9-1 were grown in flow cell reactors in EPRI media, in the continuous presence of different concentrations of cis-2-decenoic acid (cis-DA). (A) Control (media); (B) 1 nM of cis-DA; (C) 1 μM of cis-DA; (D) 1 mM cis-DA. Following 96 h of culture, biofilms were observed using a confocal microscope at a 400× magnification.
