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Antitermination of C. acetobutylicum alaS leader RNA using tRNAAla mutants. Elements ABCDE found in C. acetobutylicum tRNAAla (UGC) were sequentially introduced into B. subtilis tRNAAla (UGC), and the resulting constructs were tested for C. acetobutylicum alaS antitermination to determine RTmax and K1/2. a: ND not determined. b: The K1/2 and RTmax were calculated without the values of 0.2 and 0.5 µM tRNA.
