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. 2015 Sep 28;5(4):1567–1582. doi: 10.3390/life5041567

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Antitermination of C. acetobutylicum alaS by tRNA^Ala (UGC). The RT_max and K_1/2 for each construct were determined by in vitro antitermination assays. C. acetobutylicum tRNA^Ala (ABCDE) and B. subtilis tRNA^Ala (abcde) are circled. ND (not determined) indicates that the K_1/2 value was too high to be determined accurately.