Table 1.

The effects of C-Ras-association domain family (RASSF) proteins on mammalian Ste20-like (MST) kinases. Experiment A; FLAG-MST1 was co-expressed with FLAG-RASSF1A or FLAG-Nore1 in HEK293 cells, immunoprecipitated with FLAG-antibody, and eluted with FLAG-peptide, and used for the in vitro kinase assay using myelin basic protein as a substrate. Experiment B; Myc-MST1 or Myc-MST2 was co-expressed with FLAG-RASSF1A, RASSF2, RASSF3, RASSF4, or RASSF6, immunoprecipitated with Myc- or FLAG-antibody, and used for the in vitro kinase assay using Mps one binder kinase activator 1 (MOB1) as a substrate. When anti-FLAG-antibody was used, the co-immunoprecipitated MST1 or MST2 was evaluated. Experiment C; FLAG-MST1 was co-expressed with Myc-RASSF2 in HEK293 cells, immunoprecipitated with FLAG-antibody, and used for the in vitro kinase assay using histone H2B as a substrate. Experiment D; FLAG-MST2 was co-expressed with Myc-RASSF6 or Myc-dRASSF in HEK293 cells, immunoprecipitated with FLAG-antibody, eluted with FLAG-peptide, and used for the in vitro kinase assay using MOB1 as a substrate.

C-RASSF	Effect on MST Kinases	Ref.
RASSF1A	Inhibition the autophosphorylation of MST1 in HEK293 cells.	[20]
	Inhibition of MST1 in the in vitro kinase assay (Experiment A).	[20]
	Dissociation of Raf-1 from MST2 and promotion of the interaction between MST2 and large tumor suppressor (LATS) 1.	[21]
	Activation of MST2 in the in vitro kinase assay (Experiment B).	[8]
RASSF2	Activation of MST1 in the in vitro kinase assay (Experiment C).	[22]
	Stabilization of MST2 in MCF-7 cells.	[23]
	Activation of MST2 in the in vitro kinase assay (Experiment B).	[8]
RASSF3	No significant effect on MST2 in the in vitro kinase assay (Experiment B).	[8]
RASSF4	Inhibition of MST2 in the in vitro kinase assay (Experiment B).	[8]
Nore1	Inhibition the autophosphorylation of MST1 in HEK293 cells.	[20]
	Inhibition of MST1 in the in vitro kinase assay (Experiment A).	[20]
	The co-expression of the active Ki-Ras activates MST1 in the complex of Nore1.	[20]
	Inhibition of the dimerization and the autoactivation of MST2.	[24]
RASSF6	Inhibition of MST1 and MST2 in the in vitro kinase assay (Experiment B and D).	[8]
	Inhibition of the autophosphorylation of MST2 in HEK293 cells.	[8]
dRASSF	Inhibition of MST2 in the in vitro kinase assay (Experiment D).	[8]
	Inhibition of the autophosphorylation of MST2 in HEK293 cells.	[8]