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Different kinds of scaffolds were seeded with aligned smooth muscle sheets. Tissues stained positive for α-smooth muscle actin, indicating the maintenance of smooth muscle phenotype around all scaffolds. DAPI staining demonstrated maintenance of alignment of the cells around the scaffolds. (A) Scaffolds without fibers; (B) scaffolds with inner fibers; (C) scaffolds with outer fibers; and (D) fiber-sandwiched scaffolds. Scale bars 100 µm.
