Fig. 5.
Absence of Nox2 activation in HIF-1α-deficient mouse embryonic fibroblasts (MEFs) exposed to IH. A: Activation of HRE reporter gene expression by IH120 in wild-type (WT) MEFs and absence of this response in HIF-1α knockout (KO) MEFs. B–D: Absence of IH60-induced up-regulation of Nox2 mRNA (B) and protein (C) expression and enzyme activity (D) in KO MEFs. Mean ± SEM (n = 5) are shown. **P < 0.01; #P < 0.05; and N.S., not significant (P > 0.05) compared with normoxic controls (N).