Sensitivity of breast cancer cells to AOA and its reversal in cells depleted of GOT1/2 and GPT2. A, MTT assay of breast cancer cell lines and normal HMECs after treatment with 2 mmol/L AOA for 48 hours. B, reversal of cytotoxic effects of AOA in SUM149 cells depleted of GOT1, GOT2, and GPT2. C, RT-qPCR of GOT1, GOT2, and GPT2 mRNA in SUM149 cells depleted of the indicated genes. D, enzymatic activity of GOT1 and GOT2 after AOA treatment (2 mmol/L) shows significant inhibition following 6 hours of exposure. Each experiment was repeated three times. All statistical analysis by Student t test; two-tailed; *, P < 0.05.