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. Author manuscript; available in PMC: 2015 Dec 30.
Published in final edited form as: Clin Cancer Res. 2015 Mar 26;21(14):3263–3273. doi: 10.1158/1078-0432.CCR-14-1200

Figure 5.

Figure 5

Molecular mechanism of AOA-induced growth inhibition and death. A, RT-qPCR analysis of SUM159 and SUM149 cells showing induction of ER stress markers after 24 hours exposure to 2 mmol/L AOA (duplicates in each assay; three replicate assays). The Student t test, two-tailed *, P ≤ 0.05. B, Western blot analysis for stress markers and apoptotic marker caspase-3 comparing AOA-sensitive SUM159 with AOA-insensitive BT474 and HCC202 cell lines after 72 hours exposure to 2 mmol/L AOA. C, induction of ER stress markers and caspase-3 is reversed by addition of aspartate to SUM159 and SUM149 cells following 48 or 72 hours of treatment with AOA; bar graph, quantitation. D, schema of proposed mechanism of action of AOA in breast cancer cells.