Table 1.

Primers for PCR amplifications of porcine MSRB3

Primer Purpose	Primer	Primer sequence (5’-3’)	Product size ,bp	Annealing T,°C
Part fragment cloning	M1F	ACCAGCCACTCAACTACTGC	1152	61
	M1R	ACTTCCACCAGCAGAGCTTC
	M2F	TGGTTCTAATTGCCTCAAAGG	774	59
	M2R	GCAATGTCAGGACACCCTC
	M3F	AGCATCCAGACTATCCCAGAAG	619	59
	M3R	TACACCTCGCCATATTGACTCA
3’-RACE	3 F1	GGGGTGGACAAGGAAGATAA	-	55
	3 F2	AAAAGCTCCTCTTCTAATGC	-	55
5’-RACE	5R1	CTTTGAGGCAATTAGAACCAG	-	60
	5R2	TACAGTTGCCTTACATTCC	-	60
	5R3	CATAAAACTGCCCTCCTACT	-	-
	5R4	CACAGGCTCGCAGGGCTACT	-	-
qRT-PCR	M4F (target)	CCTCAGGGTCATGTAGGGATAAA	155	60
	M4R (target)	TCCAGGATCTTTGTGATGTGTATATTC		60
	G1F (control)	AGGGCATCCTGGGCTACACT	166	60
	G1R (control)	TCCACCACCCTGTTGCTGTAG		60
Polymorphism discovery for exons	M345F (exon 1)	CCGTGCCCAGGAATT	345	54
	M345R (exon1)	CGGCTCAGGAAAGAGG
	M278F (exon 2)	GTGTTCAGTTCCAGATAAAACC	278	53
	M278R (exon 2)	ACCCCAGACCAGTGCC
	M314F (exon 3)	GGGACTGGTCTGGTCATT	314	56
	M314R (exon 3)	CGGATTCAGCGGTTGG
	M308F (exon 4)	GCCCTTGAAAGATTTATTGG	308	52
	M308R (exon 4)	CCGGGGAGGAACAGAA
	M190F (exon 5)	TTCCCTTTGCAGGTCAG	190	59
	M190R (exon 5)	CATAGCCCTGCTTTGAGA
	M272F (exon 6)	GTATCTCCTCTGTTTTGGTTC	272	59
	M272R (exon 6)	TCATTGTGCTTGTCTGTCC
	M618F (exon 7)	CGTGGTTTCCATCGTTC	618	52
	M618R (exon 7)	CAAGCACCTTCTGCCTC
	M2F (exon 7)	TGGTTCTAATTGCCTCAAAGG	774	59
	M2R (exon 7)	GCAATGTCAGGACACCCTC
	M3F (exon 7)	AGCATCCAGACTATCCCAGAAG	619	59
	M3R (exon 7)	TACACCTCGCCATATTGACTCA
	M839F (exon 7)	GCTCCTCTTCTAATGCTTACT	839	54
	M839R (exon 7)	GCAGCCTATGGCAAACT
Polymorphism discovery for promoter	M175F	GGCTTTGGAATGAGGTTT	175	53
	M175R	TTAGACGCTGTGCTAGTTGT
	M692F	AAACAACTAGCACAGCGTC	692	50
	M692R	GCCCAAATGCCAAAA
	M694F	TTTCAGATACCCAGCATTG	694	50
	M694R	GCCTGAAGGGGAGTGTTA
	M754F	CCCATTACTGTGAGGAAAA	754	53
	M754R	AAAGCAGAGCCGAGCA
	M423F	TGGCTCGCTGTCGGA	423	58
	M423R	GGCGGGCTCATGGAA