Table 2.

List of the five genes identified by HT screening as important for the cellular fitness of high β-catenin signaling cells (HuH6) but not for low β-catenin signaling (HuH6^shCTNNB1 + Dox) cells. All siRNAs with a median RZ-scores < −2 or >2 were considered hits. Genes were considered as hits if at least two of the four siRNAs were siRNA hits, for at least two phenotypes (cell proliferation, apoptosis or mitotic events) in high β-catenin signaling cells (HuH6, HuH6 + Dox, HuH6^shCTNNB1), but not in low β-catenin signaling cells (HuH6^shCTNNB1 + Dox). The RZ-score for the best siRNA hits/gene is shown

	Cell count				Apoptosis				Mitotic events
Genes Hits	HuH6	HuH6+ Dox	HuH6shCTNNB1	HuH6shCTNNB + Dox	HuH6	HuH6+ Dox	HuH6shCTNNB1	HuH6shCTNNB + dox	HuH6	HuH6+ Dox	HuH6shCTNNB1	HuH6shCTNNB + Dox
HGS	−3.62	−3.11	−3.49		2.33	2.69	2.02				−2.11
STRADA	−2.64	−4.08	−5.1		3.15	3.11	3.62			3.31
FES	−2.63	−2.71	−2.44		2.22	2.24	2.11				−2.33
BRAF	−2.57	−2.83	−2.86		2.77	2.63	2.29
PKMYT1	−2.51	−2.21	−2.31		3.43	3.98	3.87