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. 2015 Dec 30;8:104. doi: 10.1186/s13039-015-0208-6

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© Domaschenz et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Distribution of repressive epigenetic marks across Pogona vitticeps metaphase chromosomes. Distribution of H3K27me3: (a) DAPI stained chromosomes, (b) merged image with H3K27me3 staining in green and DAPI in blue, (c) Representative line scans of staining on a macrochromosome (red) and microchromosome (yellow). The blue curves correspond to the DAPI staining along the length of the chromosomes. The green curves show the distribution of H3K27me3. d Karyotype of chromosomes depicted in image b. Distribution of H3K4me3: (e) DAPI stained chromosomes, (f) merged image showing H3K9me2 staining in green and DAPI staining in blue. g Representative line scans of staining on a macrochromosome (red) and microchromosome (yellow). h Karyotype of chromosomes depicted in image g. Scalebars represent 10 μm