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. 2015 Dec 29;16:24. doi: 10.1186/s12867-015-0052-6

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© Li et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — miR-150 affects the expression of proteins involved in cell proliferation and apoptosis. a The mRNA expression of cell cycle-related genes CyclinD1 and p27 in the three C-33A sub-cell lines (control, miR-150, miR-150 inhibitor) was measured by RT-PCR. Mean values ± SD for 3 independent experiments is shown. b The mRNA expression of apoptosis-related genes FASL and BIM in these three sub-cell lines from 3 independent experiments was evaluated by RT-PCR. Mean values ± SD for 3 independent experiments are shown. c The expression of Cyclin D1, p27, GAPDH, and phophorylated pRb (p-pRb) in the sub-cell lines was determined by western blot, and the pixel densities of these proteins were analyzed and presented by histograms (bottom). Mean values ± SD for 3 independent experiments is shown. d The expression of FASL, BIM, and GAPDH in the sub-cell lines was determined by western blot, and the pixel densities of these proteins were analyzed from 3 independent experiments and presented by histograms (bottom)