Table 1.
Primers, plasmids, and strains used in the mouse intranasal challenge model experiments
| Primer, plasmid, or strain | Relevant characteristics | Source or reference |
|---|---|---|
| Primer | ||
| FTT0482d5’ | GCTCAACATTGTATGAAATTAATGGCTCCA | Invitrogen |
| FTT0482p5’ | GTGAAATAGTCAGACAAGTAAGCCTTGGT | Invitrogen |
| FTT0482d3’ | ATTTGTATCAGCCAAATGCTGTTACGCA | Invitrogen |
| FTT0482p3’ | GTAGAATGTGGATGAATGTTAAGTACGGT | Invitrogen |
| FTT0902d5’ | CTGCTGCTGCTCAGACAGCTACTACTG | Invitrogen |
| FTT0902p5’ | GCTGCTGCTGTATCTAAGCCAACTGC | Invitrogen |
| FTT1538d5’ | CAGCAGGCGATTATGGCTACAAACA | Invitrogen |
| FTT1538p5’ | GCAGGTCAGATGTCGACACAAGAAGC | Invitrogen |
| FTT1538d3’ | TGCTTCAGCTTCGGACTTAGCAACA | Invitrogen |
| FTT1538p3’ | ATCCAATTGCTGCATCCACACCATCA | Invitrogen |
| FTT1564d5’ | CTCCTCCTCATATCAGTTCTGTCAGCT | Invitrogen |
| FTT1564p5’ | GTCGGTTTCCCAAGCTACTGGAATGT | Invitrogen |
| FTT1564d3’ | GGACTCGAACCTACGACCTACGGAT | Invitrogen |
| FTT1564p3’ | CTAACCAACTGAGCTATAGGCCCA | Invitrogen |
| FTT1597d5’ | CGGCTGATAATGATGGCTTTATGGCT | Invitrogen |
| FTT1597p5’ | TGATCCTCCTGAATATGATGATCCTAGT | Invitrogen |
| FTT1597d3’ | GCATATACGGCTGAATCTTGCCACCT | Invitrogen |
| FTT1597p3’ | CAATGATGCCAATGCCGCGGTAACT | Invitrogen |
| Plasmid | ||
| pCR2.1-TOPO | 3,931-bp TA vector; pMB1 oriR; Kmr | Life Technologies |
| pGreenhopper | pCR2.1-TOPO containing a 4-kbp Mariner-Himar 1 transposon with kanamycin resistance gene and a 1.2 kbp tnpA transposition gene. Non-replicable in F. tularensis | Unpublished |
| E. coli | ||
| TOP10 | General cloning and blue/white screening | Life Technologies |
| F. tularensis | ||
| FT12 | Type strain, a mouse-passaged isolate of a strain obtained from a U.S. patient | McKesson BioServices |