Table 3.
TLR agonists up-regulate IL-1β intracellular content at 6 and 24 h in purified rat cortical microglia and enriched, but not microglia-depleted, astrocytes
| Treatment | Microglia (pg IL-1β/well) | Astrocytes (pg IL-1β/well) | ||||
|---|---|---|---|---|---|---|
| - - - | L-LME | - - - | L-LME | |||
| 6 h | 24 h | 6 h | 6 h | 24 h | 24 h | |
| Control | 16.0 ± 1.0 | 0 | 62.6 ± 4.1 | 37.6 ± 1.3 | 66.4 ± 2.0 | 41.5 ± 2.0 |
| LPS | 1711.7 ± 63.9 | 878.1 ± 30.2 | 5150.4 ± 162.4 | 154.4 ± 26.7 | 6636.9 ± 285.5 | 155.0 ± 17.6 |
| Zymosan | 2036.2 ± 53.9 | 1382.6 ± 29.1 | 6910.8 ± 742.0 | 186.5 ± 15.8 | 7081.9 ± 405.1 | 157.3 ± 15.2 |
| Poly(I:C) | 1710.0 ± 125.1 | 631.1 ± 41.7 | 3911.5 ± 96.8 | 138.5 ± 4.3 | 3174.6 ± 151.7 | 62.6 ± 0.7 |
Purified microglia and enriched or purified (L-LME-treated) astrocytes were challenged with LPS (100 ng/ml), zymosan (10 μg/ml) or poly(I:C) (50 μg/ml) and processed after 6 and 24 h for intracellular IL-1β content by ELISA. Data are means ± SEM (n = 3). Note the lack of astrocyte responsiveness after L-LME. Values for IL-1β content of the corresponding culture media samples were <5 % of the intracellular values (not shown)