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. 2015 Dec 30;8:230. doi: 10.1186/s13068-015-0420-y

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© Shida et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — TLC Chromatogram of transglycosylation products by rBGLII. Serial amounts (5–0.075 µg by two-fold dilution) of rBGLII in 50 µL of 20 % cellobiose were subjected to TLC. The reaction mixtures were developed by silica gel plates in n-BuOH-AcOH-H₂O (3:1:1, by volume) and stained with 0.5 % thymol in EtOH/H₂SO₄. G and Sp represent Glucose and α-sophorose, respectively. L2–7 and G2–6 represent laminari-oligosaccharides and cello-oligosaccharides. Arrows represent peaks consistent with the standard substance. Open arrow heads represent unknown transglycosylation products