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. 2015 Dec 30;14:208. doi: 10.1186/s12934-015-0399-x

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© Linares et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — a Genetic map of the pACE expression vector. repC and repA, replication genes; cat, chloramphenicol resistance gene; aguR, gene encoding the regulatory agmatine-sensor-regulator protein AguR; P_aguR, aguR promoter; RBS, ribosome binding site; T1, T2 and T3, transcription terminators (ΔG = −10.3, −9.7 and −8.3 kcal/mol respectively); P_aguB*, aguB agmatine-inducible promoter carrying the A > T mutation in the fifth nucleotide of the cre site; MCS, multicloning site; HisTag; C-terminal histidine tag. Representative restriction sites are indicated. b Overview of the AguR/PaguB expression system including its components and role. AguR is the transmembrane agmatine (black filled circles) sensor and response regulator that activates P_aguB, thereby triggering the massive overproduction of the recombinant target protein