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Purification of GFP protein using the ACE system
| Step | Total protein (µg) | Protein (µg ml−1) | Total activity (U mg−1) | Protein yield (%)a |
|---|---|---|---|---|
| Lysate | 63,927 | 15,981 | 80 | |
| Flow through | 53,463 | 13,365 | 0 | |
| Wash 1 | 3244 | 811 | 0 | |
| Wash 2 | 4220 | 1055 | 0 | |
| Elution 1 | 3049 | 6098 | 0 | |
| Elution 2 | 235 | 471 | 171 | 47 |
| Elution 3 | 55 | 111 | 0 | |
| Elution 4 | 488 | 977 | 0 |
aRecovery of fluorescence activity relative to the total activity of the soluble extract
