Fig. 2.

Analysis of the isolated cells by FACS screening. a Confirmation of plasmids by agarose gel electrophoresis. Lane M represents DNA size markers (kb). Lanes 1 represents plasmid from the original cell cultivation harboring pCES-H36-GFP. Lanes 2 and 3 represent plasmids from the 1st round sorted cells and the 2nd round sorted cells. The dashed and solid arrows indicate the correct pCES-H36-GFP and IS element-inserted plasmid, respectively. b Location of IS element insertion in egfp. Gray bar indicates the open read frame of egfp. Arrows indicate the IS element insertion site. Upper and lower region represent ISCg1 and ISCg2, respectively. Double or triple triangles means double or triple clones