Fig. 3.

Inhibition of PQ-induced myofibroblast transformation by Res. WI38-VA13 cells were treated with PQ (10 µM), Res (10 µM), or PQ + Res (pretreated for 16 h) for 24 h. Immunostaining was performed with an anti-αSMA antibody followed by Alex 549-conjugated second antibody (red). A, images were taking by confocal microscopy. The nucleus was counterstained with DAPI (blue). Bars, 20 nm. B, quantification of the images taken from five representative separate fields. **, p < 0.01; ***, p < 0.001.