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. 2013 May;17(5):429–437. doi: 10.1089/gtmb.2012.0366

FIG. 1.

FIG. 1.

Analysis of JAK2V617F standards. (A) Human erythroleukemic (HEL) 92.1.7 genomic DNA was diluted in normal human peripheral blood DNA and analyzed by standard dideoxy sequence analysis. Position of the G to T transversion is highlighted by arrows. (B) Plot of the measured percentage JAK2V617F values obtained from diluted HEL 92.1.7 DNA versus expected percentage JAK2V617F values based on no copy number adjustment (blue) or with a copy number adjustment (pink). (C) Polycythemia vera (PV) patient peripheral blood DNA was diluted in normal human peripheral blood DNA and analyzed by standard dideoxy sequence analysis. Position of the G to T transversion is highlighted by arrows. The same normal control electropherogram is shown in (A) and (C).