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Reproducibility of real-time polymerase chain reaction (PCR) JAK2V617F assay. (A) Wild-type-mutant (WT-MUT) ΔCt values plotted against the log-fold ratio of JAK2V617F standards for eight independent experiments. Average values±standard deviation. (B) Percentage JAK2V617F values were determined for standards in eight independent experiments. Average values±standard deviation.
