Table 2.

Inter-Assay Variability of Standard Curve Generated with Polycythemia Vera Patient DNA and Human Erythroleukemic 92.1.7 DNA diluted in Wild-Type Genomic DNA

	Back-calculated JAK2V617F standards (%)
V6I7F standard (%)	Run 1	Run 2	Run 3	Run 4	Run 5	Run 6	Run 7	Run 8	Mean	SD	CV	%Diff
0	0	0	0	0	0	0	0	0	0	0	N/A	N/A
2	2.0	1.9	2.2	2.4	1.9	2.4	1.7	1.9	2	0.3	15.0	0
4	3.7	4.2	3.5	4.1	4.2	4.3	3.6	3.5	4	0.4	10.3	−3
8	7.1	7.5	6.9	7.2	7.5	7.5	6.5	7.4	7	0.4	5.6	−10
11	10	12	11	11	12	11	10	11	11	0.7	6.4	−1
19	17	18	17	18	18	19	19	20	18	1.1	6.0	−4
28	28	28	27	27	28	29	27	27	28	0.7	2.5	−1
38	40	38	39	40	38	40	40	40	39	1	2.5	3
47	49	49	49	47	49	46	49	47	48	1.2	2.5	3
56	55	55	55	54	55	54	54	53	54	0.7	1.3	−3
66	65	65	65	67	65	66	66	67	66	0.8	1.2	0
75	75	76	74	75	76	76	75	76	75	0.6	0.8	1
84	84	84	84	83	84	84	84	83	84	0.4	0.5	0
90	90	90	90	90	90	90	90	90	90	0.1	0.1	0
95	95	96	96	96	96	96	96	95	96	0.3	0.3	1
98	98	97	97	97	97	98	97	98	97	0.3	0.3	−1
R2	0.999	0.999	0.999	0.999	0.999	0.999	0.999	0.999	1.000