Figure 1.

Cardiac tropism of multiple AAV serotypes. (A) Structure of the AAV vector expressing GFP. (B) Representative flow-cytometric analysis of cardiac differentiated iPSC cultures transduced with AAV serotype 1. Cardiomyocytes were identified by expression of cTnT, and transduction efficiency was evaluated by GFP expression. (C) GFP-positive cells were quantitatively assessed within cardiac (cTnT+) and noncardiac (cTnT−) populations. (D) AAV transduction screening of cardiac differentiated iPSC cultures with 7 AAV serotypes (1, 2, 5, 6, 8, 9, and 10) across 3 different MOIs (100, 1000, and 10,000 vg/cell). GFP% cells were calculated based on the formula in (B) (n=1 for each condition). AAV, adeno-associated virus; CMV, cytomegalovirus; cTnT, cardiac troponin T; GFP, green fluorescent protein; iPSC, induced pluripotent stem cell; MOI, multiplicity of infection; SV40, Simian Virus 40. Color images available online at www.liebertpub.com/humc