Table 1.
Proteins identified in proteomic screens as being modified by ubiquitin or SUMO
| Initiation factor | Ubiquitin | SUMO | Reference |
|---|---|---|---|
| eIF1A | Hs Ubiquitin*Mm Ubiquitin | Rn SUMO-3 | 35,36,58,59 |
| eIF2A | Hs Ubiquitin*Mm Ubiquitin | Hs SUMO-2*At SUMO | 35,55,59,80 |
| eIF2α | Hs Ubiquitin*Mm Ubiquitin | Dm SUMO | 36,56,59 |
| eIF2B-β | Hs Ubiquitin*Mm Ubiquitin | Hs SUMO-1/2 | 36,59,81 |
| eIF2β | Hs Ubiquitin*Mm Ubiquitin | At SUMO*Sc SUMO-1 | 36,59,80,82 |
| eIF2 subunit 1 | Hs Ubiquitin*Mm Ubiquitin | Rn SUMO-3 | 35,58,59 |
| eIF2γ | Hs Ubiquitin*Mm Ubiquitin | Dm SUMO*Hs SUMO-1*Hs SUMO-2/3*Sc SUMO | 35,36,56,57,59,82,83, * |
| eIF5B* | Hs Ubiquitin*Mm Ubiquitin | Hs SUMO-2*Hs SUMO-1* | 55,59,61(A) |
| eIF3A | Hs Ubiquitin*Mm Ubiquitin*Rn Ubiquitin | Hs SUMO-2*Hs SUMO-1 | 36,55,59,61(B)* |
| eIF3B | Hs Ubiquitin*Mm Ubiquitin | Hs SUMO-2 | 35,55,59 |
| eIF3C | Hs Ubiquitin*Mm Ubiquitin | Hs SUMO 1/2 | 35,59,81 |
| eIF3D | Hs Ubiquitin*Mm Ubiquitin | Rn SUMO-3 | 35,58,59 |
| eIF3E | Hs Ubiquitin*Mm Ubiquitin | Hs SUMO-1/2 | 35,59,81 |
| eIF3F | Hs Ubiquitin*Mm Ubiquitin | 59,74(C) | |
| eIF3G | Hs Ubiquitin*Mm Ubiquitin | 35,36,59 | |
| eIF3H | Hs Ubiquitin*Mm Ubiquitin*Rn Ubiquitin | 35,36,59(D)* | |
| eIF3I | Hs Ubiquitin | Sc SUMO*Hs SUMO-1/2 | 35,36,59,60,82–84 |
| eIF3J | Hs Ubiquitin*Mm Ubiquitin | 36,59 | |
| eIF3K | Hs Ubiquitin*Mm Ubiquitin | 35,36* | |
| eIF3L | Hs Ubiquitin*Mm Ubiquitin | 35,59 | |
| eIF3M | Hs Ubiquitin*Mm Ubiquitin | Hs SUMO-1 | 35,59,83 |
| eIF3X | Hs SUMO-2 | 55 | |
| eIF4A1 | Hs Ubiquitin*Mm Ubiquitin*Rn Ubiquitin | Dm SUMO*Rn SUMO-3*Hs SUMO-1/2*At SUMO | 35,36,55–62(D) |
| eIF4A2 | Hs Ubiquitin*Mm Ubiquitin | Hs SUMO-1 | 35,59,61 |
| eIF4E | Hs Ubiquitin*Mm Ubiquitin | Hs SUMO-1 | 36,46,59 |
| eIF4GI | Hs Ubiquitin*Mm Ubiquitin | Hs SUMO-1/2 | 36,57,59,61 |
| eIF4GII | Hs Ubiquitin | 35 | |
| eIF4GIII | Hs Ubiquitin*Mm Ubiquitin | 35,36 | |
| eIF5A | Hs Ubiquitin*Mm Ubiquitin*Rn Ubiquitin | Hs SUMO-1/2 | 35,59,83(D) |
| PABP1 | Hs Ubiquitin*Mm Ubiquitin | Hs SUMO-2*Sc SUMO | 35,55,57,59,85 |
| PABP4 | Hs Ubiquitin*Mm Ubiquitin | Hs SUMO-2 | 35,55,59 |
Hs: human, Rn: rat, Mm: mouse, Sc: S. cerevisiae, At: Arabidospsis. (A) (2010) CST Curation Set: 9913; Year: 2010; SILAC: N; Biosample/Treatment: AMO-1(cell line)/Velcade; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990. (B) (2008) CST Curation Set: 3970; Year: 2008; SILAC: N; Biosample/Treatment: brain(tissue)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK. (C) (2009) CST Curation Set: 8668; Year: 2009; SILAC: N; Biosample/Treatment: RPMI-8266(cell line)/Velcade; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990. (D) (2007) CST Curation Set: 3578; Year: 2007; SILAC: N; Biosample/Treatment: brain(tissue)/ischemia and Reperfusion; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK.