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Comparison of performance parameters between HSCCC and RP-HPLC chromatography for 100 mg steroid saponins separation campaigns.
| Parameters | HSCCC | RP-HPLC | Ratio HSCCC/RP-HPLC |
|---|---|---|---|
| Run time/injection (min) | 210 | 60 | 3.5 |
| Mobile phase flow rate (mL min−1) | 2 | 16 | 0.125 |
| Operating pressure (psi) | 290 | 2896 | 0.1 |
| Sample loading/run (mg) | 100 | 100 | 1 |
| Injection volume (mL) | 10 | 2 | 5 |
| Total solvent used | 420 | 960 | 0.437 |
| Purity of five compounds | Over 95% | Over 95% | Close 1 |
| Detector | ELSD | UV | - |
